ABSTRACT
An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100 percent). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation
Subject(s)
Animals , Coturnix , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G/isolation & purification , Newcastle disease virus/isolation & purificationABSTRACT
Comparou-se o potencial patogênico de amostras vacinais virais da doença de Newcastle (VDN) e da bronquite infecciosa (VBI), através do teor hídrico traqueal (THT) de galinhas livres de anticorpos anti-VDN e anti-VBI, infectadas por via respiratória, com amostras La Sota e VG-GA de VDN e H-52 e H-120 de VBI. Maior THT foi induzido pelas amostras mais patogênicas (La Sota e H-52) do que pelas amostras menos patogênicas (VG-GA e H-120). O método determinou o potencial patogênico das amostras, sem associaçäo bacteriana no processo infeccioso
Subject(s)
Infectious bronchitis virus , Newcastle Disease , Poultry , TracheaABSTRACT
O presenta trabalho detalha o plano de assistencia a saude no Distrito Federal para o periodo de 1979-1985. A implantacao da rede basica de saude e enfatizada e sao formuladas recomendacoes sobre regionalizacao dos servicos de saude e formacao de recursos humanos basicos para o funcionamento do sistema